Porovnat metody
Prohlédněte si vybrané metody vedle sebe; řádky, které se liší, jsou zvýrazněny.
| Rezonance povrchového plazmonu× | SERS× | |
|---|---|---|
| Obor | Spektroskopie | Spektroskopie |
| Rodina | Process / pipeline | Process / pipeline |
| Rok vzniku≠ | 1971 | 1974 |
| Tvůrce≠ | Erich Kretschmann | Martin Fleischmann |
| Typ≠ | Optical technique | Vibrational spectroscopy technique |
| Původní zdroj≠ | Kretschmann, E. (1971). Determination of optical constants of metals by excitation of surface plasmons. Zeitschrift für Physik, 241(4), 313-324. link ↗ | Fleischmann, M., Hendra, P. J., & McQuillan, A. J. (1974). Raman spectra of pyridine adsorbed at a silver electrode. Chemical Physics Letters, 26(2), 163-166. DOI ↗ |
| Další názvy≠ | SPR, surface plasmon, SPR biosensing | Surface-enhanced Raman scattering, SERS spectroscopy |
| Příbuzné | 3 | 3 |
| Shrnutí≠ | Surface Plasmon Resonance (SPR) is a real-time, label-free technique for detecting and monitoring biomolecular interactions at a sensor surface by measuring changes in the refractive index caused by ligand binding. Developed by Kretschmann in 1971 and applied to biosensing by Liedberg, Nylander, and Lundström in 1983, SPR is now a gold standard for measuring binding kinetics (association and dissociation rates) and equilibrium binding constants in protein interactions, antibody-antigen recognition, and drug discovery. | Surface-Enhanced Raman Spectroscopy (SERS) amplifies weak Raman signals by many orders of magnitude when analyte molecules are adsorbed on specially prepared metal (typically silver or gold) nanostructured surfaces. Discovered by Fleischmann, Hendra, and McQuillan in 1974, SERS enables detection of vibrational signatures of single molecules and ultra-trace contaminants, revolutionizing analytical chemistry and forensics. |
| ScholarGateDatová sada ↗ |
|
|