Porovnat metody
Prohlédněte si vybrané metody vedle sebe; řádky, které se liší, jsou zvýrazněny.
| Izotermická titrační kalorimetrie× | Elektronová paramagnetická rezonance× | |
|---|---|---|
| Obor | Spektroskopie | Spektroskopie |
| Rodina | Process / pipeline | Process / pipeline |
| Rok vzniku≠ | 1989 | 1945 |
| Tvůrce≠ | Terrence Wiseman | Evgeny Zavoiskii |
| Typ≠ | Biophysical technique | Spectroscopic technique |
| Původní zdroj≠ | Wiseman, T., Williston, S., Brandts, J. F., & Lin, L. N. (1989). Rapid measurement of binding constants and heats of binding using a new titration calorimeter. Analytical Biochemistry, 179(1), 131-137. DOI ↗ | Zavoiskii, E. K. (1945). Paramagnetic relaxation of liquid solutions for perpendicular fields. Zhurnal Eksperimental'noi i Teoreticheskoi Fiziki, 15(6), 378-380. link ↗ |
| Další názvy | ITC, isothermal calorimetry, microcalorimetry | EPR spectroscopy, ESR, electron spin resonance |
| Příbuzné | 3 | 3 |
| Shrnutí≠ | Isothermal Titration Calorimetry (ITC) is a thermodynamic technique that measures heat released or absorbed during biomolecular binding events at constant temperature. Developed by Wiseman and colleagues in 1989, ITC directly determines binding affinity (Kd), enthalpy (ΔH), and entropy (ΔS) in a single experiment, making it one of the most comprehensive methods for characterizing molecular interactions without requiring labels or immobilization. | Electron Paramagnetic Resonance (EPR), also called Electron Spin Resonance (ESR), is a spectroscopic technique that detects and characterizes unpaired electrons in molecules and materials. Discovered by Zavoiskii in 1945, EPR measures the absorption of microwave radiation by paramagnetic species in a magnetic field, providing information about electron spin states, local electronic environment, and molecular dynamics. |
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