Porovnat metody
Prohlédněte si vybrané metody vedle sebe; řádky, které se liší, jsou zvýrazněny.
| Imunochemický test inhibice hemaglutinace× | Enzyme-Linked Immunosorbent Assay (ELISA)× | |
|---|---|---|
| Obor | Veterinární vědy | Veterinární vědy |
| Rodina | Process / pipeline | Process / pipeline |
| Rok vzniku≠ | 1942 | 1971 |
| Tvůrce≠ | George K. Hirst | Eva Engvall and Peter Perlmann; independent parallel development by Anton Schuurs and Bauke van Weemen |
| Typ≠ | Serological diagnostic assay | Quantitative immunoassay |
| Původní zdroj≠ | Hirst, G. K. (1942). The quantitative determination of influenza virus and antibodies by means of red cell agglutination. Journal of Experimental Medicine, 75(1), 49–64. DOI ↗ | Engvall, E., & Perlmann, P. (1971). Enzyme-linked immunosorbent assay (ELISA) quantitative assay of immunoglobulin G. Immunochemistry, 8(9), 871–874. DOI ↗ |
| Další názvy | HI assay, HAI test, haemagglutination inhibition test, HI test | enzyme immunoassay, EIA, solid-phase enzyme immunoassay, ELISA test |
| Příbuzné≠ | 1 | 2 |
| Shrnutí≠ | The Hemagglutination Inhibition (HI) Assay is a classical serological test used to detect and quantify antibodies against hemagglutinating viruses — most notably influenza and Newcastle disease virus — in animal and human serum. Widely employed in veterinary diagnostics, vaccine efficacy evaluation, and epidemiological surveillance, it relies on the principle that specific antibodies in serum will block a known quantity of virus from agglutinating red blood cells. | ELISA is a plate-based immunoassay technique that detects and quantifies proteins, antibodies, antigens, hormones, and other analytes in biological samples. Widely used in veterinary science, medicine, and food safety, it exploits the specificity of antibody–antigen binding coupled to an enzyme-driven colorimetric signal to deliver sensitive, reproducible measurements across large sample batches. |
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