قارن الطرق

	تحليل السلالات الخلوية المفردة	تحليل تسلسل الحمض النووي الريبوزي للخلايا المفردة
المجال	المعلوماتية الحيوية	المعلوماتية الحيوية
العائلة	Process / pipeline	Process / pipeline
سنة النشأة	2014-2020 (rapid development period)	2009 (first scRNA-seq by Tang et al.); widely adopted 2015–2016
صاحب الطريقة	Multiple groups; foundational tools: Trapnell et al. (Monocle, 2014), Jones et al. (Cassiopeia, 2020)	Azim Surani, Barbara Treutlein, and the Regev/McCarroll groups (foundational droplet-based methods ~2015)
النوع	Computational phylogenetic inference pipeline	High-throughput single-cell transcriptomic profiling pipeline
المصدر التأسيسي	Jones, M. G., Khodaverdian, A., Quinn, J. J., Chan, M. M., Hussmann, J. A., Wang, R., Xu, C., Weissman, J. S., & Yosef, N. (2020). Inference of single-cell phylogenies from lineage tracing data using Cassiopeia. Genome Biology, 21(1), 92.	Satija, R., Farrell, J. A., Gennert, D., Schier, A. F., & Regev, A. (2015). Spatial reconstruction of single-cell gene expression data. Nature Biotechnology, 33(5), 495–502.
الأسماء البديلة	scPhylogeny, single-cell lineage tracing, clonal phylogenetics, single-cell tree inference	scRNA-seq, single-cell transcriptomics, scRNAseq analysis, single-cell gene expression profiling
ذات صلة	4	5
الملخص	Single-cell phylogenetic analysis reconstructs evolutionary or developmental trees from single-cell sequencing data, tracing how individual cells diverged from a common ancestor. By leveraging somatic mutations, CRISPR-introduced barcodes, or copy-number changes as heritable characters, this method maps clonal relationships within tumors, developing tissues, or immune repertoires with unprecedented cellular resolution.	Single-cell RNA sequencing (scRNA-seq) analysis characterises gene expression at the resolution of individual cells, enabling discovery of cell types, states, and transitions that are invisible in bulk transcriptomics. Starting from raw sequencing reads, the workflow produces a cell-by-gene count matrix and proceeds through quality control, normalisation, dimensionality reduction, unsupervised clustering, cell-type annotation, and a range of downstream analyses such as trajectory inference and differential expression between cell populations.