قارن الطرق

	تحليل eQTL للخلايا المفردة	تحليل تسلسل الحمض النووي الريبوزي للخلايا المفردة
المجال	المعلوماتية الحيوية	المعلوماتية الحيوية
العائلة	Process / pipeline	Process / pipeline
سنة النشأة	2020	2009 (first scRNA-seq by Tang et al.); widely adopted 2015–2016
صاحب الطريقة	Cuomo et al.; Kim-Hellmuth et al. (pioneering sc-eQTL frameworks, 2020)	Azim Surani, Barbara Treutlein, and the Regev/McCarroll groups (foundational droplet-based methods ~2015)
النوع	Statistical genomics pipeline	High-throughput single-cell transcriptomic profiling pipeline
المصدر التأسيسي	Cuomo, A. S. E., et al. (2020). Single-cell RNA-sequencing of differentiating iPS cells reveals dynamic genetic effects on gene expression. Nature Communications, 11(1), 810.	Satija, R., Farrell, J. A., Gennert, D., Schier, A. F., & Regev, A. (2015). Spatial reconstruction of single-cell gene expression data. Nature Biotechnology, 33(5), 495–502.
الأسماء البديلة	sc-eQTL analysis, single-cell eQTL mapping, scRNA-seq eQTL, cell-type-specific eQTL	scRNA-seq, single-cell transcriptomics, scRNAseq analysis, single-cell gene expression profiling
ذات صلة	6	5
الملخص	Single-cell eQTL analysis identifies genetic variants (eQTLs) that regulate gene expression in a cell-type-specific manner by jointly analysing single-cell RNA-seq profiles and donor genotype data. Unlike bulk eQTL methods, it resolves regulatory effects that are diluted or masked when cell types are mixed, enabling discovery of variants whose effects are confined to particular cell states or developmental stages.	Single-cell RNA sequencing (scRNA-seq) analysis characterises gene expression at the resolution of individual cells, enabling discovery of cell types, states, and transitions that are invisible in bulk transcriptomics. Starting from raw sequencing reads, the workflow produces a cell-by-gene count matrix and proceeds through quality control, normalisation, dimensionality reduction, unsupervised clustering, cell-type annotation, and a range of downstream analyses such as trajectory inference and differential expression between cell populations.