قارن الطرق
راجع الطرق التي اخترتها جنبًا إلى جنب؛ الصفوف المختلفة مميَّزة.
| تحليل البروتينات: توصيف البروتينات المستند إلى قياس الطيف الكتلي× | تحليل التعبير التفاضلي لتسلسل الحمض النووي الريبوزي (RNA-seq DE)× | |
|---|---|---|
| المجال | المعلوماتية الحيوية | المعلوماتية الحيوية |
| العائلة | Process / pipeline | Process / pipeline |
| سنة النشأة≠ | 1994–2003 (term coined 1994; shotgun proteomics established early 2000s) | 2008–2010 (RNA-seq DE methodology established) |
| صاحب الطريقة≠ | Marc Wilkins, Matthias Mann, Ruedi Aebersold (proteome/mass spectrometry foundations) | Multiple groups; foundational methods from Anders & Huber (DESeq, 2010), Robinson, McCarthy & Smyth (edgeR, 2010) |
| النوع≠ | Quantitative omics pipeline | Quantitative genomics pipeline |
| المصدر التأسيسي≠ | Wilkins, M. R., Sanchez, J.-C., Gooley, A. A., Appel, R. D., Humphery-Smith, I., Hochstrasser, D. F., & Williams, K. L. (1996). Progress with proteome projects: Why all proteins expressed by a genome should be identified and how to do it. Biotechnology and Genetic Engineering Reviews, 13(1), 19–50. link ↗ | Love, M. I., Huber, W., & Anders, S. (2014). Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biology, 15(12), 550. DOI ↗ |
| الأسماء البديلة | proteomics, mass spectrometry-based proteomics, shotgun proteomics, quantitative proteomics | RNA-seq DE analysis, transcriptomic differential expression, bulk RNA-seq DE, DEA |
| ذات صلة | 6 | 6 |
| الملخص≠ | Proteomics analysis is a systematic pipeline for identifying and quantifying proteins in biological samples using mass spectrometry. Starting from raw spectral data, the workflow searches protein sequence databases, estimates abundance across conditions, applies statistical tests for differential expression, and maps findings onto biological pathways. It complements transcriptomics by capturing post-translational regulation and actual protein abundance, and is central to biomarker discovery, drug-target identification, and systems biology. | RNA-seq differential expression (DE) analysis identifies genes whose transcript abundance differs significantly between two or more biological conditions — for example, treated versus control, or diseased versus healthy tissue. Starting from raw sequencing reads, the pipeline moves through alignment, count-based normalization, statistical modeling of count dispersion, hypothesis testing, and multiple-testing correction to produce a ranked list of differentially expressed genes accompanied by fold-change estimates and adjusted p-values. |
| ScholarGateمجموعة البيانات ↗ |
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