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تحليل قياس التدفق الخلوي×الفيزيولوجيا الكهربائية بتقنية الالتقاط الرقعي (Patch-Clamp Electrophysiology)×
المجالعلم الأدويةعلم الأدوية
العائلةProcess / pipelineProcess / pipeline
سنة النشأة19761976
صاحب الطريقةLeonard HerzenbergErwin Neher and Bert Sakmann
النوعcell analysis and sortingion channel screening
المصدر التأسيسيHerzenberg, L. A., Parks, D., Sahaf, B., Perez, O., Roederer, M., & Herzenberg, L. A. (2002). The history and future of the fluorescence-activated cell sorter and flow cytometry: a view from Stanford. Clinical Chemistry, 48(10), 1819-1827. DOI ↗Neher, E., & Sakmann, B. (1976). Single-channel currents recorded from membrane of denervated frog muscle fibres. Nature, 260(5554), 799-802. DOI ↗
الأسماء البديلةFACS, fluorescence-activated cell sorting, cell analysispatch clamp, whole-cell recording, ion channel assay
ذات صلة33
الملخصFlow cytometry is a laser-based technology for analyzing and sorting individual cells based on fluorescent markers. Developed by Leonard Herzenberg in the 1970s, flow cytometry enables rapid assessment of cell phenotype, drug effects on cell populations, and therapeutic cell characterization in immunology and hematology.Patch-clamp electrophysiology is a technique for measuring ionic currents through ion channels in cell membranes, developed by Neher and Sakmann in 1976. It enables direct observation of single-channel and whole-cell currents at millisecond resolution, making it essential for characterizing drug effects on ion channels and cardiac safety assessment.
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ScholarGateقارن الطرق: Flow Cytometry · Patch-Clamp. استُرجع بتاريخ 2026-06-15 من https://scholargate.app/ar/compare