قارن الطرق

	فحص نفاذية خلايا كـاكـو-2	الفيزيولوجيا الكهربائية بتقنية الالتقاط الرقعي (Patch-Clamp Electrophysiology)
المجال	علم الأدوية	علم الأدوية
العائلة	Process / pipeline	Process / pipeline
سنة النشأة	1989	1976
صاحب الطريقة	Ingrid Hidalgo	Erwin Neher and Bert Sakmann
النوع	absorption screening	ion channel screening
المصدر التأسيسي	Hidalgo, I. J., Raub, T. J., & Borchardt, R. T. (1989). Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability. Gastroenterology, 96(3), 736-749.	Neher, E., & Sakmann, B. (1976). Single-channel currents recorded from membrane of denervated frog muscle fibres. Nature, 260(5554), 799-802.
الأسماء البديلة	Caco-2 assay, intestinal permeability, ADME screening	patch clamp, whole-cell recording, ion channel assay
ذات صلة	3	3
الملخص	The Caco-2 assay is an in vitro model system using human colon carcinoma cell monolayers to screen drug intestinal permeability. Developed by Hidalgo and colleagues in 1989, Caco-2 cells differentiate into an epithelial barrier resembling intestinal mucosa, enabling rapid assessment of drug absorption potential and identification of transporter-mediated transport.	Patch-clamp electrophysiology is a technique for measuring ionic currents through ion channels in cell membranes, developed by Neher and Sakmann in 1976. It enables direct observation of single-channel and whole-cell currents at millisecond resolution, making it essential for characterizing drug effects on ion channels and cardiac safety assessment.