Imaging Mass Cytometry
Imaging Mass Cytometry (IMC) is a multiplexed proteomics technique that maps the subcellular localization of up to 40-50 proteins in tissue sections simultaneously using mass spectrometry detection. Developed by Bodenmiller and colleagues in 2014, IMC combines the single-cell imaging power of immunofluorescence with the multiplexing capacity of mass cytometry, enabling comprehensive analysis of cell types, states, and spatial interactions within tissue microenvironments. IMC has emerged as a powerful tool in immuno-oncology, immunobiology, and tissue biology for dissecting cellular heterogeneity and spatial organization.
Rekodi ya chanzo
Nukuu zimehamishwa kwa uhalisi kutoka kwa rekodi ya chanzo cha mbinu. Hakuna uthibitisho wa kiwango cha dai unaodokezwa kutoka kwao.
- Giesen, C., Wang, H. A., Schapiro, D., et al. (2014). Highly multiplexed imaging of tumor tissues with subcellular resolution by mass cytometry. Nature Methods, 11(4), 417-422. · DOI 10.1038/nmeth.2869
- Jackson, H. W., Fischer, J. R., Zanotelli, V. R., et al. (2020). The single-cell pathology of synovial inflammation in rheumatoid arthritis. Nature Medicine, 26(6), 941-951. · URL
- Schulz, D., Zanotelli, V. R., Fischer, J. R., et al. (2018). Simultaneous multiplexed imaging of mRNA and proteins with subcellular resolution in breast cancer tissue samples by imaging mass cytometry. Nature Protocols, 13(12), 2825-2848. · DOI 10.1016/j.cels.2018.04.004
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