GPC/SEC
Gel permeation chromatography (GPC), also known as size exclusion chromatography (SEC), is an analytical technique for determining the molecular weight distribution (MWD) and average molecular weight (Mw, Mn) of polymers. The method separates polymer molecules by their hydrodynamic size as they pass through a porous chromatography column: larger molecules elute first (excluded from pores), while smaller molecules are retained longer. Developed by Moore and colleagues in the 1960s, GPC/SEC is now the standard method for characterizing polymer chains, assessing polymer degradation over time, and verifying batch consistency in biomaterial production.
Allikakirje
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- Striegel, A. M., Yau, W. W., Kirkland, J. J., & Bly, D. D. (2009). Modern size-exclusion liquid chromatography: practice and theory. John Wiley & Sons. · URL
- Podzimek, S. (2011). Light scattering, size exclusion chromatography and asymmetric flow field flow fractionation: promising tools for the characterization of polymers and nanoparticles. John Wiley & Sons. · URL
- Bateman, L. C., & Moore, C. G. (2017). Determination of molecular weight and molecular weight distribution. In The Chemistry and Physics of Rubber-Like Substances. Academic Press. · URL
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