Σύγκριση μεθόδων

	Ανάλυση Κυτταρομετρίας Ροής	Δοκιμασία Διαπερατότητας Κυττάρων Caco-2
Πεδίο	Φαρμακολογία	Φαρμακολογία
Οικογένεια	Process / pipeline	Process / pipeline
Έτος προέλευσης	1976	1989
Δημιουργός	Leonard Herzenberg	Ingrid Hidalgo
Τύπος	cell analysis and sorting	absorption screening
Θεμελιώδης πηγή	Herzenberg, L. A., Parks, D., Sahaf, B., Perez, O., Roederer, M., & Herzenberg, L. A. (2002). The history and future of the fluorescence-activated cell sorter and flow cytometry: a view from Stanford. Clinical Chemistry, 48(10), 1819-1827.	Hidalgo, I. J., Raub, T. J., & Borchardt, R. T. (1989). Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability. Gastroenterology, 96(3), 736-749.
Εναλλακτικές ονομασίες	FACS, fluorescence-activated cell sorting, cell analysis	Caco-2 assay, intestinal permeability, ADME screening
Συναφείς	3	3
Σύνοψη	Flow cytometry is a laser-based technology for analyzing and sorting individual cells based on fluorescent markers. Developed by Leonard Herzenberg in the 1970s, flow cytometry enables rapid assessment of cell phenotype, drug effects on cell populations, and therapeutic cell characterization in immunology and hematology.	The Caco-2 assay is an in vitro model system using human colon carcinoma cell monolayers to screen drug intestinal permeability. Developed by Hidalgo and colleagues in 1989, Caco-2 cells differentiate into an epithelial barrier resembling intestinal mucosa, enabling rapid assessment of drug absorption potential and identification of transporter-mediated transport.