eDNA Metabarcoding
Environmental DNA (eDNA) metabarcoding detects and identifies species present in environmental samples (water, soil, air) by sequencing short DNA fragments released by organisms. Developed by Taberlet and colleagues (2012), this approach has revolutionized biodiversity monitoring: species can be surveyed without capture, observation, or complex sampling designs. Metabarcoding sequences millions of DNA fragments, identifies reads taxonomically, and assigns them to species. The method is non-invasive, rapid, and cost-effective, enabling large-scale biodiversity surveys and early detection of cryptic or rare species.
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- Taberlet, P., Coissac, E., Hajibabaei, M., & Rieseberg, L. H. (2012). Environmental DNA. Molecular Ecology, 21(8), 1789-1793. · DOI 10.1111/j.1365-294X.2012.05542.x
- Deakin, G., Pettitt-Wade, H., & Waldick, R. C. (2016). Environmental DNA metabarcoding: A review of the application to fish biodiversity assessment in temperate freshwaters. Environmental DNA, 1(1), 4-14. · URL
- Ficetola, G. F., Miaud, C., Pompanon, F., & Taberlet, P. (2008). Species detection using environmental DNA from water samples. Biology Letters, 4(4), 423-425. · DOI 10.1098/rsbl.2008.0118
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